METHOD FOR THE PURE CULTIVATION OF PATHOGENIC TREPONEMA PALLIDUM (SPIROCHÆTA PALLIDA)

Spirochæta (treponema) Pallida and Syphilis

The extensive bibliography on experimental syphilis and spiroch~eta pallida has been brought together and reviewed recently by Hoffmann, 1 Neisser, 2 R. Kraus, 3 Glass, 4 and Buschke and Fischer, 5 in whose publications the present state of our knowledge on these subjects can be readily found. The weight of opinion is strongly in favor of accepting the transmissibility of the syphilitic virus t...

The Direct Cultivation of Treponema Pallidum Pathogenic for the Monkey

A method for the direct cultivation of Treponema pallidum from human syphilitic lesions, by the employment of a solid medium, has been described. By means of it, three of the four strains worked with were successfully cultivated. The several pure cultures agree in morphological and cultural characters, grow only in the presence of sterile tissue under anaerobic conditions, and do not produce pu...

Spirochæta Pallida (Spironema Pallidum) in Syphilis

Pure Cultivation of Spirochæta Refringens

A strain of Spirochoeta refringens has been obtained in pure culture, and its morphological and cultural characteristics have been studied. The strain possessed no pathogenicity for rabbits or monkeys. It probably belongs to the genus Treponema, but it is easily differentiated from Treponema pallidum, Treponema microdentium, and Treponema macrodentium. Its relation to Spirochoeta balanitidis an...

The Pure Cultivation of Spirochæta Duttoni, Spirochæta Kochi, Spirochæta Obermeieri, and Spirochæta Novyi

1. A method for the pure cultivation of Spirochoeta duttoni, Spirochoeta kochi, Spirochoeta obermeieri, and Spirochoeta novyi is described in this paper. In vitro these strains reach their maximum growth after seven, eight, or nine days at 37 degrees C. For their multiplication they require the presence of a piece of fresh sterile tissue and a body fluid capable of forming a loose fibrin with t...